Soybeans will be grown in soil uniformly infested with Races 3, 5 or 14 of the Soybean Cyst Nematode Heterodera glycines. Once female cysts are present on the roots (30 days) the number of cysts on plant roots will be counted and compared to the number of cysts on susceptible check ( Cosroy 79, Williams 82 or Lee). We grow four replications for each entry and include the standard variety in each replication. Results have been consistent on check varieties.
Cost for this test will be $29.00 per race per variety entered or $7.25 per replication per entry if more than four replications are desired.
If it is helpful to breeders we can maintain plant that appear resistant in these tests and take them to maturity in the greenhouse in order to harvest seed.
Seeds are germinated and then
transplanted into pasteurized soil.
About 3,000 Heterodera glycines
eggs are pipetted into each cone.
Watch a video describing the setup for the SCN screening.
Watch a video describing how cysts are removed from plants after 30 days.
Watch a video of cysts being dislodged from roots.
Watch a video of cysts being counted in the laboratory.