Soybeans will be grown in pasteurized soil and a uniform number of SCN eggs will be added to each cone (plant). We have different SCN populations depending on the source of resistance one is looking for. Some populations reproduce effectively on PI 88788 another on Peking and another is easily managed by any source of resistance. Once female cysts are present on the roots (30 days) the number of cysts on plant roots will be counted and compared to the number of cysts on susceptible check (Williams 82 or Lee 74). We grow four replications for each entry and include the standard variety in each replication. Results have been consistent on check varieties.
Cost for this test will be $30.00 per race per variety entered or $7.50 per replication per entry if more than four replications are desired.
If it is helpful to breeders we can maintain plant that appear resistant in these tests and take them to maturity in the greenhouse in order to harvest seed.
Seeds are germinated and then
transplanted into pasteurized soil.
About 3,000 Heterodera glycines
eggs are pipetted into each cone.
Watch a video describing the setup for the SCN screening.
Watch a video describing how cysts are removed from plants after 30 days.
Watch a video of cysts being dislodged from roots.
Watch a video of cysts being counted in the laboratory.